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    歡迎來合肥萊爾生物科技有限公司!我們將為您提供周到的服務!銷售熱線:0551-66107970 15375214453
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    聯系我們Contact us
    公司名稱:合肥萊爾生物科技有限公司
    聯系人:陳經理
    固話:0551-66107970
    手機:15375214453
    地址:合肥市包河經濟開發區花園大道17號

    產品名稱:膳食纖維檢測試劑盒

    產品規格:100 assays / 200 assays

    產品貨號:K-TDFR-100A,K-TDFR-200A

    產品報價:

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    Content: 100 assays / 200 assays
    Shipping Temperature: Ambient
    Storage Temperature: Short term stability: 2-8oC,
    Long term stability: See individual component labels
    Stability: > 2 years under recommended storage conditions
    Analyte: Dietary Fiber
    Assay Format: Enzymatic
    Detection Method: Gravimetric
    Signal Response: Increase
    Limit of Detection: 0.5 g/100 g
    Total Assay Time: ~ 100 min
    Application examples: Food ingredients, food products and other materials.
    Method recognition: AACC Method 32-05.01, AACC Method 32-06.01, AACC Method 32-07.01, AACC Method 32-21.01, AOAC Method 985.29, AOAC Method 991.42, AOAC Method 991.43, AOAC Method 993.19, CODEX Method Type I and GB Standard 5009.88-2014

    The Total Dietary Fiber Assay Kit for the analysis of Total, Soluble and Insoluble Dietary Fiber according to AOAC and AACC approved methods.

    View Dietary Fiber Measurement Guide - Which Method for which sample?

    Dietary fiber can generally be described as the carbohydrate content of food that is not digested in the human small intestine. It passes into the large intestine where it is partially or fully fermented. These characteristics of dietary fiber are associated with its numerous well documented health benefits.

    Dietary Fiber is a mixture of complex organic substances, including hydrophilic compounds, such as soluble and insoluble polysaccharides and non-digestable oligosaccharides, as well as a range of non-swellable, more or less hydrophobic, compounds such as cutins, suberins and lignins. The procedures for the determination and analysis of total dietary fiber as outlined in our assay protocol are based on the methods of Lee et al.1 and Prosky et al.2,3 (AOAC 991.43, AOAC 985.29, AACC 32-07.01 and AACC 32-05.01). However, the enzymes in the Megazyme Total Dietary Fiber Kit can also be used in other dietary fiber analytical methods such as AACC Method 32-21.01 and AACC Method 32-06.01.

    1. Association of Official Analytical Chemists. (1985). Official Methods of Analysis, 14th ed., 1st suppl. Secs. 43, A14-43, A20, p.399.
    2. Association of Official Analytical Chemists. (1986). Changes in methods. J. Assoc. Off. Anal. Chem., 69, 370.
    3. Association of Official Analytical Chemists. (1987). Changes in methods. J. Assoc. Off. Anal. Chem., 70, 393.

    See General Referee Reports: Journal of AOAC INTERNATIONAL, Vol. 81, No. 1, 1998. 

    Two separate methods are described in the associated assay protocol:

    METHOD 1: DETERMINATION OF TOTAL, SOLUBLE AND INSOLUBLE DIETARY FIBER
    Based on AOAC Method 991.43 “Total, Soluble, and Insoluble Dietary Fiber in Foods” (First Action 1991) and AACC Method 32-07.01 “Determination of Soluble, Insoluble, and Total Dietary Fiber in Foods and Food Products” (Final Approval 10-16-91).

    METHOD 2: DETERMINATION OF TOTAL DIETARY FIBER
    Based on AACC method 32-05.01 and AOAC Method 985.29.

    Note that a letter of endorsement from the original method developer, Dr. Leon Prosky, is included in the Documents Tab.

    Scheme-K-TDFR-200A TDFR Megazyme

    See our full range of starch and dietary fiber products.

    Advantages
    • Very competitive price (cost per test)   
    • All reagents stable for > 2 years 
    • High purity / standardised enzymes employed 
    • Mega-Calc? software tool is available from our website for hassle-free raw data processing 
    • Simple format

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